2, 4- dichlorophemoxy acetic acid (2, 4-D) is a selective herbicides. It is widely used as a weed killer. The half-life of 2, 4-D in the environment is relatively short, averaging 10 days in soils and less than 10 days in water. Its low concentrations can also stimulate RNA, DNA and Protein Synthesis leading to uncontrolled cell division and growth. On the other hand, high concentration of 2, 4-D can inhibit cell division and growth. Plant death typically within 3-5 weeks for Reagents 2, 4-D solutions are prepared, for this, stock solutions of 1mg/ml of 2, 4-D was prepared in concentrated H2SO4. A working standard of 40 µg/ml also prepared. 6-Amino-1-naphtol-3 Sulfonic acid (J-acid) solution (Wilson) was prepared by 0.4% solution in concentrated sulphuric acid. A 50% ammonium acetate solution was prepared in Distilled water lowing applications. 2, 4-D shows acute and non-acute effects. Determination of 2, 4-D effluent in water sample, grains like Maize, Rice and plants parts was taken for experiments and its effect were recorded. It affects badly on animal health and plants also. So, the limited amount like 0.7ppm can be allowed.
