The screening and study of selected Indian medicinal plant Wrightia tinctoria R.Br., were selected for phytochemical screening and antibacterial studies. The solvents used for the extraction of plant roots were ethanol, benzene, chloroform, acetone, petroleum ether and distilled water. The Gram-Postive and Gram-negative bacteria Yeast candida, Aspergillus niger, Staphylococcus aureus, Eschericha coli, Salmonella typhi, Bacillus subtilis, Pseudomonas fluorescence, Klebsiella pneumonia and Streptococus pyogenes. were tested. The results obtained in the present study suggest that preliminary phytochemical analysis detected the presence of Alkaloids, Flavonoids, Terpenoids, Steroids, Cumarins, Carbohydrates and Tanins. The Wrightia tinctoria R. Br. could be used in treating diseases caused by the test organisms.