The present investigation was carried out to study the effect of different growth regulators on propagation of firebush (Hamelia patens Jacq.) in subtropical zone of West Bengal under naturally ventilated polyhouse equipped with foggers at Instructional Farm, Jaguli, Bidhan Chandra Krishi Viswavidyalaya, West Bengal. Significant differences among the treatments (IAA @1000ppm, IAA@2000ppm, IAA @3000ppm, IBA @1000ppm, IBA @2000ppm, IBA @3000ppm, NAA @1000ppm, NAA @2000ppm, NAA @3000ppm and Control) on root emergence and proliferation were noticed. After two consecutive years of studied, this plant can successfully propagate by tip cutting with IAA @ 3000ppm followed by IBA @ 3000ppm and NAA @ 1000ppm for rapid multiplication of this plant in sub-tropical zone of West Bengal during rainy season.