Environmental and biodiversity risks were greatly increased by toxic heavy metal pollution, The various water samples environmental with a history of heavy metal toxicity resulting from human activities or natural origin released in the environment. Ensure representative sampling from various depths and locations within the fields. Their widespread distribution in the environment as a result of their numerous industrial, household, agricultural, medical, and technical applications has sparked worries about their possible consequences on the environment and public health. Bioluminescent bacteria are thought to be an essential and effective tool. The luminous bacteria for Waste water, Seawater, Tap water and monitoring of different toxicants was to be isolated, optimized, and applied. The Microtox assay is frequently employed as the initial screening method in a test battery to identify microbial toxicity. From various source it is reported that a heavy metal toxicity monitoring efficacy of Vibrio harveyi has the ability to detect pollution in distinct environmental and various water conditions. It is anticipated that the Vibrio sp. Could be succeed in toxicity detection at different environmental and various salinity conditions. The history of bacterial bioluminescence discoveries and its present uses in environmental research will be reviewed, with a focus on the Microassay and luminometer methods.
