The environmental concerns due to increased oil consumption, comes a solution for the development of CI engines which are used as alternative fuels. However, there is need of a fundamental study of mixture and spray formation for a fuel under various conditions. The spray characteristics of the fuel greatly influence emissions from diesel engines. Spray development plays a vital role in controlling the combustion and emission characteristics of engine because it directly affects the air fuel mixture. The spray characteristic of fuel mainly depends on fuel injection pressure, fuel density, fuel viscosity, ambient pressure and temperature. The present work is to examine spray formation in high pressure chamber through optical access. The material used to fabricate the high pressure spray chamber is Cast Iron (CI) and two optical glass fitted perpendicular to each other to capture the spray image inside chamber by using high speed camera. The analysis focused on spray characteristic on spray pattern and spray area. The viscosity effect and chamber pressure leads to change in formation of spray parameters. Spray tip penetration length decrease when increase in chamber pressure. Some other parameters like width, cone angle and area increase when there is an increase in chamber pressure. The spray cone angle increased as the chamber pressure increases for diesel and palm oil methyl ester (POME).