A case of cross breed cow milk having problem of curdling of milk on incubation was reported in the polyclinics of college of Veterinary Science and Animal Husbandry, U. P. Pt. Deen Dayal Upadhyay Veterinary University and Go Anusandhan Sansthan,Mathura, U. P. India and milk samples were send to the Department of Livestock Products Technology of the same university. The chemical composition of milk was normal and the health status of cow was also normal. The case was suspected for lack of calcium contents. So the three treatments were given to the milk during traditional routine curdling process. The samples in which calcium chloride was added gave normal curd whereas; other treatments showed the problem of curdling and no curd was formed in such treatments. It indicates that calcium is having significant role in the curd formation.