Introduction: It has been suggested that vitamin C deficiency is associated with gingival inflammatory changes; however, the disorder is infrequently encountered in the time. But there are many cases of extensive gingival overgrowth caused by vitamin C deficiency associated with multifactorial causes, which needed vitamin C supplement. Material and Method: The clinical examination included intraoral examination with blood specimen for testing serum vitamin C to get more learning about gingival enlargement. Results, Discussion, conclusion: In this study, the actual level at which vitamin C in patients group was founded lower than the normal or reference vales in comparison to control group and this was explained in the table below. The aesthetic and functional difficulties are the most causes of gingival overgrowth. There are many causes for gingival overgrowth, but the etiopathogenesis cause was usually associated with factors such as the use of certain drugs or systemic disorders such as vitamin C deficiency. The patients have moderate vitamin C deficiency and the local factors like plaque and calculus may be associated with the pathogenesis of severe gingival overgrowth.