In this study, commercially available four different bio-fertilizers have been used. 1) Cocktail mychorrhiza, which contained 9 species of mychorrhiza; Glomus aggregatum, Glomus clarum, Glomus deserticola, Glomus etunicatum, Glomus intraradices, Glomus mosseae, Glomus monosporus, Glomus brasilianum, Gigaspora margarita, 2) Bacteria (Seranade) which contained Basillus subtilis, 3) Bacteria (Roa Natura) which contained Arthrobactersp, 4) Microalgae (Allgrow) which contained Chlorella spp., and chemical fertilizers used in conventional farming were applied to the control plants. Plants were grown in pots in a controlled medium to avoid contamination. The lettuce plants grown under different treatments were compared at the end of the study; the yield, head circumference, diameter and height dimensions of the product, fresh and dry weights, chlorophyll content and the macro-and micro-nutrient element contents were investigated. As a result, with the use of bio-fertilizers in the plants, they benefited more from the nutrient elements efficiently. The addition of beneficial microorganisms to the soil in greenhouse cultivation affected the yield and quality positively.