An in vitro regeneration protocol was developed for Lysimachia laxa Baudo. Maximum number of shoot (9.10±0.32) was induced by culturing nodal explants in Murashige and Skoog (MS) medium supplemented with 17.76 µM benzyladenine (BA) and 5.37 µM α – naphthalene acetic acid (NAA). Elongated shoots were best rooted, with 100% rooting efficiency, in MS medium containing 2.85 µM indole-3-acetic acid (IAA). Among the two explants (nodal and shoot tips) used, nodal explants were found to be the best option for in vitro regeneration of Lysimachia laxa. Rooted plantlets thus developed were hardened in 2 months time by growing in root trainers containing potting mixture of a 1:1 mix of garden soil and leaf mould. The established plants were then transferred to earthen pots and subsequently transferred to field condition.
