The aim of this study was to characterize the bacterial profile of Diabetic foot Infections (DFI) and Urinary Tract infections (UTIs) and to assess the antibiotic sensitivity of the causative pathogens. Specimens were obtained from 254 Lebanese patients (109 with DFI and 145 with UTI).Bacterial identification and antibiotic susceptibility of E. coli isolates from those patients were assessed using the Vitek machine. Staphylococcus coagulase. Negative (33.03%) and E.coli (27.52%) were the most common isolates identified in DFIs. On the other hand, E. coli (73.10%) and Klebsiella (11.03%) were the two most frequent pathogens detected in UTIs. E. coli isolates from DFIs and UTIs showed comparable antibiotic sensitivity profiles with being significantly sensitive to ertapenem, meropenem, imipenem or amikacin, only moderately susceptible to fluoroquinolones (ciprofloxacin and levofloxacin) and weakly sensitive to ceftazidime, cefepime and bactrim. The etiology of DFIs and UTIs as well as their susceptibility patterns are essential for the Public health in Lebanon where antibiotics misuse is serious.