ß-Glucuronidase is the novel enzyme known to present in the tissues of several vertebrates. It can be detected by biochemical, histochemical and immunocytochemical techniques in bacteria, plant and animal. The traditional method used for histochemical demonstration of ß-glucuronidase is quite tedious and substrate utilized in a given concentration is not affordable to researchers of the developing country. In the present study the attempt is made to improve the localization of ß-glucuronidase by employing the same method with change in substrate concentration and by avoiding the use of some chemicals. It was observed that under the light microscope the enzyme can be localize clearly both in the granular and diffuse form without any artifact. This modified method could be beneficial for the localization of ß-glucuronidase in other animals beside mammals and fishes.
