In order to develop the control signal for electrocardiograph (ECG), the property, forms, size and numeric parameters of the control signal shall be identified. In doing so, a mathematical processing and analysis were done on the electrocardiograms used as the input signal of the ECG because we supposed that the signal for checking normal condition of the equipment shall have the same rate and the same property with the equipment’s input signal. We aimed to select the most suitable option of control signals by making calculations using Fourier’s transformation. Up to date, the researches and studies on the electrocardiograms have been done only for clinical analysis, but it is insufficient today as the development of equipment and technology has reached much higher level. Thus, a feature-length study of electrocardiogram signals using special software has been started. In this study, while performing mathematical processing of the electrocardiograms, we used Mathcad for Fourier’s transformation.