A total of 225, 16 weeks old layer birds randomly divided into 4 groups with three replicates each. Birds were assigned to the basal diet (control). All birds employed in the experiment were fed according to applicable recommendations of the National Research Council Feed and water bacterial count assessed before and after completion of trial. Fresh purified herbal extracts of turmeric, fenugreek and ginger were subjected to sterility and in vitro antibacterial effect test. The herbal ingredients were incorporated to the basal diet at the level of 0.1%, 0.25% and 0.5% respectively. First week of feeding showed reduction of anaerobic bacterial count when compared with untreated control group. Second and third week of herbal feeding showed significance difference of anaerobic bacterial excretion in dropping materials compared to untreated control group. Simultaneously growth rate and feed conversion ratio also improved in the test groups when compared with untreated control group. Feeding of crude preparation of fenugreek, Ginger, turmeric along with basal diet at the level of 0.1%, 0.25% and 0.5% had significance influence on the reduction of excretion of fecal anaerobic bacteria and no changes in excretion of beneficial bacteria and feeding of herbal extract do not any negative impact on body weight and blood parameters.
